Question 1

What is CryoProcess?

Accepted Answer

CryoProcess is a web-based graphical user interface for RELION cryo-EM data processing with live processing capabilities. Built with Node.js and React, it provides a browser-accessible frontend that submits and monitors RELION jobs on your SLURM cluster in real time.

Question 2

How is CryoProcess different from running RELION directly?

Accepted Answer

CryoProcess does not replace RELION. It wraps RELION in a modern web interface with live processing updates. All processing is done by RELION running in Singularity containers on your cluster. CryoProcess adds real-time job monitoring, project management, visual pipeline tracking, and SLURM job configuration through the browser.

Question 3

What versions of RELION are supported?

Accepted Answer

CryoProcess fully supports RELION 5 as well as earlier versions. RELION runs via Singularity containers. You provide the container with your preferred version, giving you full control.

Question 4

What operating systems are supported?

Accepted Answer

Linux (Ubuntu 20.04+, Debian, CentOS, RHEL). CryoProcess runs on the server; users access it from any OS via web browser.

Question 5

Do I need root access to install?

Accepted Answer

Root access is needed for installing system dependencies (MongoDB, Node.js). The application itself runs as a regular user.

Question 6

What if I don't have a SLURM cluster?

Accepted Answer

SLURM integration is a core feature. If you are running on a single workstation, you would need a minimal SLURM configuration.

Question 7

Can I install CryoProcess on a cloud VM?

Accepted Answer

Yes. Any Linux VM with SLURM configured can run CryoProcess. Configure the ALLOWED_HOSTS setting with your VM's IP address.

Question 8

Can multiple users access CryoProcess simultaneously?

Accepted Answer

Yes. CryoProcess uses JWT-based authentication. Each user logs in with their own credentials and manages their own projects.

Question 9

What external tools can I integrate?

Accepted Answer

CTFFIND, MotionCor2, and ResMap. Configure their paths in the .env file and CryoProcess will integrate them into your pipeline.

Question 10

Where is my data stored?

Accepted Answer

Processing data stays on your cluster at the path specified by ROOT_PATH. Job metadata is stored in a local MongoDB database. Your data never leaves your infrastructure.

Question 11

Is CryoProcess free?

Accepted Answer

Yes. CryoProcess is completely free for both academic and industry users. All features are included with no usage limits.

Question 12

How do I get support?

Accepted Answer

All users can file issues on GitHub or email support@cryoprocess.com. If you need help for installation and support, please contact support@cryoprocess.com.

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