Use Cases & Workflows
See how CryoProcess fits into your cryo-EM research.
Single-Particle Analysis Lab
A structural biology lab running daily cryo-EM collections on a shared microscope. Multiple lab members need to process datasets independently.
How CryoProcess helps:
- Live processing with real-time job monitoring and result preview
- Multi-user authentication for independent project management
- Web access from any workstation in the lab
- SLURM integration for fair cluster scheduling
Researchers can start processing data on day one through an intuitive web interface.
Shared HPC Facility
A university computing center supports multiple cryo-EM labs with a shared SLURM cluster and Singularity-based software stack.
How CryoProcess helps:
- Singularity container deployment for consistent environments
- Configurable bind paths for each lab's data directories
- SSH bridge for remote cluster submission
- Centralized installation with per-user access
Facility administrators deploy once; all labs access via browser.
Remote Collaboration
A research group with members at multiple institutions needs to process and review cryo-EM data collaboratively.
How CryoProcess helps:
- Live processing updates visible from any browser
- Browser-based access with no local installation required
- JWT authentication for secure remote access
- Shared project pipelines across team members
Collaborators can review processing results without needing cluster access or software installed locally.
Teaching & Training
A graduate course or workshop on cryo-EM methods needs students to run RELION processing jobs hands-on.
How CryoProcess helps:
- Simplified interface lowers the barrier to entry
- Default configurations minimize setup mistakes
- One installation serves an entire class
- Visual pipeline helps students understand processing flow
Students can run processing jobs and view results immediately through an intuitive web interface.
A Typical Processing Session
Walk through a complete single-particle analysis workflow in CryoProcess.
Create a New Project
Start by creating a project and specifying the directory containing your raw micrograph movies.
Import Micrograph Movies
Import your raw movie files into the project. CryoProcess detects file formats automatically.
Run Motion Correction
Correct for beam-induced specimen motion using RELION's own implementation or MotionCor2.
Estimate CTF
Determine the contrast transfer function parameters using CTFFIND for each micrograph.
Pick Particles
Identify and extract individual particle images from the motion-corrected micrographs.
2D Classification
Sort particles into classes to remove junk and identify different views of your molecule.
Generate Initial Model
Create a starting 3D reference from your best 2D class averages.
3D Classification & Refinement
Separate structural heterogeneity and refine the best class to high resolution.
Post-Processing
Apply B-factor sharpening, masking, and resolution estimation to your final map.
Ready to try it with your data?
Install CryoProcess and run your first processing job today.